A New-Cell free Liquid Medium for Rapidly Culturing Mycobacterium le praemurium
ND-5-Medium
1975 年 51 巻 9 号 p. 707-711
詳細
1975 年 51 巻 9 号 p. 707-711
A cell-free liquid medium (ND-5) for M. lepraemurium was recently established. In this medium, Dubos medium was employed in lieu of Kirchner medium in the case of NC-5 medium. When M. lepraemurium was inoculated into ND-5 medium, the number of bacilli rapidly increased approximately 10 to 30-fold for one week's incubation. A possible generation time can be calculated as 1.4-2.6 days. Furthermore, morphological features indicated that the cells grown in ND-5 medium were significantly solider than those in NC-5 medium.
