The banding patterns of the chromosomes of Hynobius tokyoensis Tago are obtained by the treatment of Sumner's BSG method with some modifications. These patterns enable the identification of 17 out of 28 pairs of chromosomes in this species. Morphologically similar chromosome pairs such as nos. 4 and 5, nos. 6 and 7, and nos. 10, 11 and 12 could be easily distinguished from one another.
