Homogeneous Hyoscyamine 6β-Hydroxylase from Cultured Roots of Hyoscyamus niger

Abstract

Hyoscyamine 6β-hydroxylase (EC 1.14.11.11), the first enzyme in the biosynthetic pathway leading from hyoscyamine to scopolamine, was purified to homogeneity from cultured roots of Hyoscyamus niger. The enzyme is a monomer of 38kDa with pI 4.6. Its amino acid composition is also reported.