Scallop striated tropomyosin prepared by a new method

Abstract

A large amount of tropomyosin was quickly prepared from scallop striated muscle by the method combining ammonium sulfate fractionation in the presence of Mg²⁺-ATP and subsequent dialysis. The yield of tropomyosin by this method was about four times higher than that by conventional method. The tropomyosin thus prepared showed essentially the same activity as ordinary preparation, e.g., it depressed the Mg²⁺-ATPase activity of rabbit skeletal actomyosin by 25%, whereas no effect on that of recom stituted scallop actomyosin was observed. This new method for preparing tropomyosin characterized by its simplicity and rapidity is expected to be applicable to different kinds of muscle.