Phage proteinに関する研究 (III)

P22Phage蛋白のアミノ酸組成と3成分の分画について

抄録

Further investigations were performed on P22-protein (Coat protein of P22 phage). Amino acid components were estimated with P22-protein by the aid of automatic amino acid analyzer. Constitutive protein fractions were analyzed by polyacrylamide gel electrophoresis. Fractionation of the 3 components, found in PAGE electrophoresis, were successfully performed with DEAE-cellulsoe column chromatography. Results were summarized as follows. 1.P22-protein is composed of alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, leucine, isoleucine, lysine, methionine, phenyl-alanine, proline, serine, threonine, tyrosine and valine and the molar ratio of them were 9, 4, 16, 8, 11, 1, 6, 4, 4, 2, 3, 1, 6, 6, 2, and 9, per mg protein, respectively. 2.Three components were detected from P22-protein in polyacrylamide gel electrophoresis (PAGE). 3.Average relative migration of the 3 components in PAGE were 0.767, 1.199 and 1.426, respectively, when that of bovine serum albumin (BSA) was 1.000. 4.Three components were successfully separated by DEAE-cellulose column chromatography. 5.Elution was performed in following conditions, i.e., 0.5g of DEAE-cellulose was packed in 10mm (diameter) × 300mm (length) glass tube and 5ml of protein solution (2.6mg/ml) was loaded, then elution was carried out with 0.001M, 0.005M and 0.02M phosphate buffer pH. 8.8. The last buffer was added with NaCl to 0.14M. The flow rate of the buffers was 0.5ml per minute. 6.Migration of the 3 fractions, corresponding to each 100 ml of buffers, coincided well to those of the 3 components detected in PAGE.