Abstract

Studies on the Phage Protein. (IV) Antibody Production with P22 Coat Protein. Michiko HORIKOSHI and Kiyoaki KAMIJO Tissue Culture Lab. ndo University, School of Medicine. Immunological activities of the P22-protein was described previously. Comparative study was performed on the immune response of the P22-protein, original P22 phage and host protein (LT 2 protein). Experiments were carried cut with rabbits. Results obtained were as follows ; 1. In a neutralizing antibody response, it must be emphasized that the P22-protein seemed to be equivalent to the original P22 phage. 2. In process of the antibody production, however, some difference was seen between anti-P22-protein- and anti-P22 -sera, suggesting the existence of some different factor in P22-protein from the original phage. It may be the degenerative product in degradation process or the changes in tertiary or quatery structure of the coat protein. 3. In animals injected with LT 2 protein (host protein), the antibody produced was quite different from those against P22 or P22-protein. The host protein should be different nature of the phage protein in immunological activity. 4. For the neutralizing antibody production, the necessary and enough doses of antigens were 5 x 10¹¹-1.15 X 10¹² for P22 phage and 2.0-2.63 mg for P22 protein, respectively. These doses should be given in 4 or 5 times.