Abstract
Using paraffin embedded blocks of resected cholecystic cancer prepared from 39 patients, the nuclear DNA content was measured by a single-cell isolation method through epi-illumination microfluorophotometry to investigate the nuclear DNA ploidy pattern in cholecystic cancer.
The nuclear DNA ploidy pattern in 17 patients with cholecystic cancer was classified into a diploid pattern and that in 22 patients an aneuploid pattern. Nuclear DNA ploidy pattern was found to be significantly correlated with lymph node infiltration, perineural infiltration, and lymph node metastasis.
The patients in the aneuploid pattern group had a significantly poorer outcome than those in the diploid pattern group. Among the patients demonstrating similar histopathological features, those in the aneuploid pattern group also had a poorer outcome than those in the diploid pattern group.
These findings suggested that the nuclear DNA ploidy pattern is effective as parameter of the outcome of cholecystic cancer.
