Interleukin-1 activity in urine of patients with bladder tumor

Abstract

Interleukin-1 in urine samples from patients with bladder carcinoma was measured to investigate the correlation between interleukin-1 and urothelial neoplasmas. Nineteen patients with bladder carcinoma, 2 patients with cystitis, 2 patients with hematuria for benign prostatic hypertrophy and 6 healthy donors participated in this study. Urine specimens were clarified by centrifugation at 2000×G for 30 min and were concentrated 10-fold and 50-fold on PM-10 membrane. Enzyme-linked immunosorbent assays were used for the analysis of interleukin-1. The interleukin-1 titers in the urine of patients with cystitis and hematuria for benign prostatic hypertrophy were increased compared to those of healthy donors. Therefore, urine specimens from patients with bladder carcinoma only were separated from those who had cystitis and hematuria as well. The interleukin-1 titers in the urine of patients with bladder carcinoma were increased compared to those of healthy donors and progressed with tumor size. Although the interleukin-1 producing site has not been elucidated yet, our study suggests that interleukin-1 is produced by the tumor cells.