抄録
Effects of fixation and nuclear staining methods for yeasts were studied comparatively. Test strains were S. cerevisiae, S. fragilis, S. rouxii, H. anomala, H. schneggii, H. saturnus, Sc. ludwigii, C. albicans, and C. robusta shown in table 1.
Results obtained were summerized as follows.
1) Buin's fluid, Carnoy's fixative and Schaudinn's fixative were excellent in nuclear staining procedure. Fixation were carried out 10 minutes in each fixative and followed by 70% alcohol treatment for 10 minutes in the case of former two kinds of fixatives. Washing by tap water was found to be enough for 10 minutes. In these procedure any debris seen in the case of Helly's fixative was not found on the surface of the specimen.
2) For the observation of the details of nuclei, DeLammater's stain was excellent, but formal-dehyde treatment was seemed to be unnecessary.
3) In some of the test organisms unknown masking substance, which could not be removed by HCl or HClO4 treatment was found. For these strains over 1 hour hydrolysis by HCl gave no discernible nuclear configurations, but cells were stained diffusely red by acid-fuchsin. At the same condition, the specimen were not stained by pyronine, which stains specifically RNA.
Pepsin digestion test was carried out by Lillie's technic to these specimen and perfect digestion of the masking substance was resulted.
By the results of the cytochemical studies on the substance, its nature is not RNA and seemed to be protein-like substance.
4) The unknown masking substance was obseved in S. cerevisiae 0635, S. cerevisiae 0637, Sc. ludwigii 0339, C. robusta 0718 and C. robusta 0735.
