Abstract
The mechanisms of development and progression of acute pancreatitis have been reported to involve escape of pancreatic enzyme from the pancreatic gland and/or decreased blood flow in the pancreatic tissue. Therefore, preventing escape of pancreatic enzyme from the pancreatic gland and/or maintaining the blood flow of the pancreatic tissue might prevent the development and progression of acute pancreatitis.
This study estimated the efficacy of pancreatic enzyme inhibitor and vaso-dilator in preventing aggravation of acute pancreatitis. To evaluate the efficacy in caerulein-induced pancreatitis in rats, octreotide acetate (SMS201-995), a somatostatin analog, was used as the pancreatic enzyme inhibitor and beraprost sodium (TRK100), a stabilized derivative of prostaglandin I2, was used as the vaso-dilator to maintain blood flow.
Male Wistar rats (approximately 250g of body weight) were administered 20μg of caerulein intraperitoneally to induce pancreatitis.
After 2.5μg of TRK100 was orally administered, caerulein was given to 5 rats. Another 5 rats with caerulein-induced pancreatitis were administered 2μg of SMS201-995.
Six hours after treatment, pancreatic tissue blood flow, serum amylase, serum elastasel, serum trypsin and drywet ratio of the pancreatic tissue were determined. Moreover, acinar cells were examined by electronanmicroscopy to estimate cell damage using original classification system.
The results indicated that TRK100 and SMS201-995 can reduced the severity of acute pancreatitis both serologically and histologically.
It is concluded that the inhibition of exocrine to prevent escape of pancreatic enzyme and maintain pancreatic tissue blood flow may prevent the development and progression of acute pancreatitis.
