2004 Volume 50 Issue 1 Pages 68-76
Background : Occurrence of antiretrovirals-resistant HIV-1 raises public health concerns in North America and Europe. Objective : To investigate genotypic resistance to antiretrovirals in HIV-1 infected individuals. Patients : Ten HIV-1-infected patients who received treatment between February 2003 and October 2003 at Juntendo University Hospital. Method : Genotypic HIV-1 resistance assay using RT-PCR and direct sequencing. Results : Three of 5 patients on therapy had resistance-associated mutations for NRTIs (D67N, K70R, M184V, T215N/V and/or K219Q), 1 had mutations for NNRTIs (K103N and V108I) and 2 had them for PIs (D30N or I84V). Two of the subjects showed resistance to two or more classes of antiretrovirals. NRTI-resistant patients tended to have a longer history of NRTI treatment, although it was not statistically significant. Two of 5 naïve patients had resistance for NRTIs (T215E) and 1 showd resistance for NNRTIs (G190A). Conclusions : Our study suggests that it is important for patients receiving treatment for a long period, those with detectable viral load, and those intending to start treatment to be tested by assay for resistance to antiretrovirals.
