Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
Molecular epidemiological characterization of 46 Vancomycin-intermediate Staphylococcus aureus strains isolated worldwide
JIN GXUNJINTERUYO ITOKEIICHI HIRAMATSU
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2011 Volume 57 Issue 5 Pages 494-503

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Abstract
Vancomycin-intermediate S. aureus (VISA) shows decreased susceptibility to vancomycin with a minimum inhibitory concentration (MIC) value of 4-8mg/l and is refractory to treatment with vancomycin, and VISA strains have appeared in many parts of the world. We conducted a molecular epidemiological study using 46 VISA strains, 43 MRSA and 3 Methicillin-susceptible Staphylococcus aureus (MSSA), isolated from 11 countries. Nine VISA clones, which belonged to four clonal complexes, CC5, CC8, CC30 and CC45, were identified as follows : CC5-typell SCCmec (19 strains), CC8-typelll SCCmec (10 strains), CC8-typeI SCCmec (6 strains), CC8-typeIVd SCCmec (3 strains), CC30-typeIl SCCmec (2 strains), CC45-typelI SCCmec (2 strains), CC8-typelI SCCmec (1 strain), CC5 (2 strain) and CC8 (1 strain). The coagulase types and the agr types of these strains were well correlated to MLST. In this study, we investigated the correlations between the spa-type and the region encoding IgG binding domain in the protein A molecule, and found that the structure of the IgG binding domain was well correlated to the MLST genotype, but there was a difference in the numbers of units in the IgG binding region among strains that belonged to a clonal complex. Based on this finding, we further analyzed the structure of protein A of the 19 CC5-type II SCCmec strains together with Pulsed Field Gel Electrophoresis (PFGE), and the carriage of the toxic shock syndrome toxin-1 gene (tst). As results, we identified two groups of clones in the United States (US) with the same banding pattern in PFGE and the same spa type, which did not carry the tst gene, suggesting the dissemination of these clones throughout US. In contrast, CC5-type II SCCmec strains from Asian countries showed marked differences in spa type, structure of IgG binding domain, and the presence or absence of tst. Our data suggest that the VISA clone has not disseminated throughout the world, but rather these clones have emerged from MRSA strains by independently accumulating mutations and are being disseminated at hospitals in the respective countries.
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© 2011 The Juntendo Medical Society
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