Abstract
Many methods have been used to classify the three species of Brucella. For instances, to measure the metabolism in nitrogen and dextrose, to recognize the reduction of nitrate and nitrit, to observe the production of H2S, or to test the resistance of the organisms against several dyes, these methods were most commonly practised in many laboratories.
Among the several methods, the dye-test which reported by Huddleson was one of the most useful method in classifying Brucella, and the author eliminated some inconveniences in this method by the application of the modified Szybalski's agar-gradient plate.
In the author's method, it was able to observe the resistance of the organisms at the more dilluted point of concentration, consuming fewer culture media.
