Abstract
The author conducted feeding tests to determine the biological effect of eosine with D.D. strain of mice ond rabbits.
The following resutts were obtained :
1.LD50 Test : The LD50 was 5.491g/kg and the 95% reliable range was3.621 to 8.322g/kg
2.Blood Concentration : Maximum blood concentration and urine excretion values were ohtained 4-6 hours after oral admininstration. Traces were still evident in the blood after 5 days and 5.2% of the amount administered were excreted in the urine after 5 days. Continous feeding for 3 days revealed that blood concentration increased with the number of feedings and that it was stored in the body.
3.Body weight Changes : Decrease in body weight was noticed in the group fed with eosine.
4.Blood Changes : Daily feeding of eosine resulted in a significant decrease in the number of erythrocytes, hemoglobin and blood sugar values, but no changes were found in the number of leukocytes, specific gravity of the whole blood, Plasma and inorganic elements of the serum. Nageli's toxic granules were observed in the leukocyte picture after two weeks of feeding which increased with the length of feeding and covered 31% of the field after 6 weeks of feeding.This phenomenon is signiicant in addition to the changes found in the number of erythrocytes and blood sugar value.
5.Urine Changes : Analyais of the urine showed no significant changes in the sugar and urobilinogen.
Proteinuria was found in the group fed eosine which clearly shows that the kidneys were ffected.
6.Organotropism : Significant pathological changes were found in the kidney. Acute nephrotic tissue changes were foudd.
The aomodnt of congestion of the kidney and haemosiderosis of the spleen makes it difficult to conclnde that eosine was responsible.
No Significant changes were found in the other organs.
