Abstract
A new, efficient and reproducible in vitro propagation system of Telopea speciosissima has been established. Excised receptacle disks were cultured for inducing adventitious shoots on modified woody plant media (WPM) supplemented with 6-benzyladenine (BA) and 3-indole-butyric acid (IBA) at various concentrations. Efficient shoot regeneration was obtained when explants were cultured on media containing 5-10mg l-1 BA and 0-0.2mg l-1 IBA. Most of the shoots were regenerated directly from the epidermis of the lateral side, and in some cases, from the cut surface of the receptacle disk explants. Nodal explants taken from the regenerated shoots were cultured on the modified WPM supplemented with a wide range of BA concentrations. The number of shoots produced from the nodal explants increased linearly until 0.3mg l-1 BA, where it then plateaued. The shoot length and leaf number were negatively correlated with increased BA concentration. Shoots were rooted by dipping their base into a solution of 150mg l-1 IBA for 10 minutes and then inserting them into Kanuma soil.
