Anthocyanin production in adventitious root cultures of Raphanus sativus L. cv. Peking Koushin

Abstract

The adventitious roots of Raphanus sativus L. cv. Peking Koushin were established by culturing root segments of in vitro seedlings in 1/2 Murashige and Skoog (MS) liquid medium supplemented with 0.5 mg/l IBA. The adventitious roots cultured in 1/2 MS liquid medium supplemented with 0.5 mg/l IBA produced anthocyanin in the dark. When cultured under the 14 h/day light condition, the adventitious roots cultured with 0.5 mg/l IBA produced more anthocyanin than those cultured with 0.1 or 0.5 mg/l NAA. The acid hydrolysis of the extract prepared from the adventitious roots revealed that the main anthocyanidin was pelargonidin. In addition, the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity of the adventitious root extract was comparable to that of roots of the intact plant grown in the field.