Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Original Papers
High throughput metabolome and proteome analysis of transgenic rice plants (Oryza sativa L.)
Hideyuki TakahashiYuji HottaMitsunori HayashiMaki Kawai-YamadaSetsuko KomatsuHirofumi Uchimiya
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2005 Volume 22 Issue 1 Pages 47-50


We profiled metabolic patterns of transgenic rice organs and tissue by Fourier-transform ion cyclotron mass spectrometry (FT-MS) to reveal effects of the over-expression of YK1 possessing high homology with maize HC-toxin reductase gene. Comparison of metabolic patterns revealed that compositions of organ- or tissue-specific metabolites were not significantly varied between the control and the YK1 rice, where expression levels of some metabolites were altered. Proteome analysis of cultured cells over-expressing YK1 showed the up-regulation of several stress-related proteins such as osmotin-like protein and osr40c1. Thus, alteration of metabolites as well as proteins may contribute to multiple stress tolerances in transgenic YK1 rice.

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© 2005 by Japanese Society for Plant Biotechnology
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