Efficient Plant Regeneration by Smashing Callus in Rice (Oriza sativa L.) Cell Culture

Abstract

Smashing of callus before regeneration in rice (Oriza sativa L.) cell culture was studied for the purpose of increasing plant regeneration efficiency. Using a blender was efficient as the method for smashing callus. The culture in hormone free medium for a week after smashing was efficient for decreasing the browning of callus and increasing the number of regenerated plantlets. In the culture using bioreactor with aeration of enriched carbon dioxide (5%), 9700 plantlets were obtained. The number of plantlets regenerated from the callus which size were 1000-1700 and 1700-2360μm were much more than that regenerated from another size of callus. From this result, more efficient regeneration would be obtained, if a method of smashing the callus uniformly into those sizes could be developed.