Abstract
We studied on the efficient culture of celery (F1) callus. Celery callus showed the best growth at 25°C. Initial medium pH in the range between 5.0 and 6.5 had little relation to callus growth. Sucrose was better than glucose as a carbon source and a 3% concentration of sucrose was the best condition. In batch culture, ammonium ion disappeared from the medium at an early stage of the culture. Therefore it could be suggested that ammonium nitrogen was one of the limitting factors of callus growth. We tried a fed batch culture of ammonium nitrogen by controlling the medium pH, the growth rate of callus in the fed batch culture was 1.45 times higher than the batch culture.
