Isolation, Culture and Thallus Regeneration of Protoplasts from the Hornwort Anthoceros punctatus L. Cultured Cells

Abstract

Protoplasts, in which regenerated plants were reported for the first time, were isolated from cultured cells of the hornwort Anthoceros punctatus. Like the parent plant the callus and thallus cells from these protoplasts possess a large chloroplast. The chloroplasts in the cultured protoplasts began to divide after 2 days from the start of culture, followed by the first protoplast cell division after 4 days.