Arabidopsis root cells stained with Kakshine PC1

Abstract

An Arabidopsis root cultured for 9 days was stained with a new DNA saining dye based on N-aryl pyrido cyanine backbone (PC1), designated Kakshine PC1, at 1 µM. The stained root was observed by two-photon excitation microscoy (LSM780-DUO-NLO; Zeiss) excited at 1000 nm using a LD C-Apochromat 40x/1.10 water immersion objective lens. It should be noted that Kakshine PC1 penetrate deep root region and stained mitochondrial DNA as well as nuclear DNA.