Identification and Characterization of a novel mitochondrial histone-like protein in Physarum polycephalum

Narie Sasaki

doi:10.5685/plmorphol.16.61

Narie Sasaki

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Keywords: Glom, mitochondrial nucleoid, histone-like protein, HMG-box

JOURNAL FREE ACCESS

2004 Volume 16 Issue 1 Pages 61-70

DOI https://doi.org/10.5685/plmorphol.16.61

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Published: 2004 Received: - Available on J-STAGE: June 28, 2010 Accepted: - Advance online publication: - Revised: -
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Date of correction: June 28, 2010 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: June 28, 2010 Reason for correction: - Correction: KEYWORD Details: Wrong : Key Words: Glom, mitochondrial nucleoid, histone-like protein, HMG-box
Right : Glom, mitochondrial nucleoid, histone-like protein, HMG-box

Date of correction: June 28, 2010 Reason for correction: - Correction: CITATION Details: Wrong : E
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Abstract

Summary: Mitochondrial DNA(mtDNA)is packed into highly organized structures called mitochondrial nucleoids(mt-nucleoids). We identified and characterized a new mitochondrial histone-like protein, termed Glom(a protein inducing agglomeration of mitochondrial chromosome), from highly condensed mt-nucleoids of the true slime mold, Physarum polycephalum. This protein has a lysine-rich region with proline-rich domain in the N-terminal half and two HMG boxes in C-terminal half. Glom induced intensive DNA condensation without suppressing replication and transcription. The lysine-rich region was sufficient for the mtDNA condensation and the proline-rich domain was essential to keep those genetic activities. The expression of Glom also complemented the E.coli mutant lacking the bacterial histone-like protein HU and the HMG-boxes region of Glom was important for the complementation.

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