γ-Tubulin in Bryophytes

Yoshinobu Mineyuki; Masaki Shimamura

doi:10.5685/plmorphol.16.71

Yoshinobu Mineyuki, Masaki Shimamura

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Keywords: evolution, γ-tubulin, basal land plant, microtubule, MTOC, spindle

JOURNAL FREE ACCESS

2004 Volume 16 Issue 1 Pages 71-82

DOI https://doi.org/10.5685/plmorphol.16.71

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Published: 2004 Received: - Available on J-STAGE: June 28, 2010 Accepted: - Advance online publication: - Revised: -
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Date of correction: June 28, 2010 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: June 28, 2010 Reason for correction: - Correction: KEYWORD Details: Wrong : Key words: evolution; γ-tubulin; basal land plant; microtubule; MTOC; spindle.
Right : evolution, γ-tubulin, basal land plant, microtubule, MTOC, spindle

Date of correction: June 28, 2010 Reason for correction: - Correction: CITATION Details: Wrong : E
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Abstract

Summary: γ-Tubulin is a protein associated with microtubule organizing centers(MTOCs)such as centrosomes in animal cells. Although seed plants have γ-tubulin, they do not have discrete MTOCs comparable to centrosomes, and organization of the multiple microtubule arrays in plants has been enigmatic. Because basal land plants have an exceptional variety of unique morphologically discrete MTOCs(e. g. centrosome, polar organizer, plastid-based quadripolar microtubule system), microtubule systems in basal land plants may hold clues to evolutionary changes in γ-tubulin localization and function in evolution of acentriolar spindles in plants. In the present study, we isolated and characterized a genomic clone encoding γ-tubulin of a liverwort(Bryophyta)and verified the localization of γ-tubulin on several types of discrete MTOCs in bryophytes using the G9 anti-γ-tubulin antibody that recognized ca. 55kDa peptide of bryophytes. We also describe changes in the localization of γ-tubulin closely correlated with the MTOC cycle in meiosis of the liverwort, Dumortiera hirsuta. γ-Tubulin is detectable on the surface of isolated plastids and nuclei and microtubules can be repolymerized from the isolated plastids. γ-Tubulin localization on plastid and nuclear surfaces are not affected by destruction of microtubules by oryzalin. From these results, we conclude that γ-tubulin is a highly conserved protein associated with microtubule nucleation in basal land plants, and it has cell cycle dependent manner essential for the orderly succession of microtubule arrays.

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