SYNTHESIS OF HYDROXYAPATITE PARTICLES INTENDED FOR THE SELECTIVE ADSORPTION OF BASIC PROTEINS

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In this study, we report a simple method for synthesizing HAp particles intended for the selective adsorption of basic proteins. HAp particles for the adsorption of basic proteins were prepared from a mixture of calcium acetate and diammonium hydrogen phosphate solutions, both adjusted at pH 10 (HAp-pH10) and a non-adjusted pH (HAp-none). For HAp-none, the amount of bovine serum albumin (BSA, an acidic protein) adsorbed per unit specific surface area was larger than the amount of lysozyme (LSZ, a basic protein) adsorbed. The ratio of the amounts of protein adsorption, i.e., LSZ/BSA, was 0.32 for HAp-none. The amount of LSZ adsorbed on HAp-pH10 was larger than those for BSA. The ratio of LSZ/BSA was 2.38 on HAp-pH10 aged for 1 h. An increase in the aging time (72 h) caused an increase in LSZ/BSA to 3.17.