抄録
We aimed to evaluate the ectopic bone formation of porous calcium-phosphate ceramics with different compositions and porous structures following implantation into porcine femoral muscle to understand the factors contributing to osteoinduction. Porous hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) ceramics were fabricated by firing calcium-phosphate fiber mixed with carbon beads. Three types of porous calcium-phosphate ceramics were fabricated for animal testing: porous HAp ceramics with 150 μm carbon beads (HAp(150)), and porous β-TCP ceramics with either 20 μm or 150 μm carbon beads (TCP(20) and TCP(150)). Individual porous calcium-phosphate ceramics were implanted into porcine femoral muscle. After 13 weeks, histological evaluation was performed. Ectopic bone formation was observed in HAp(150) and TCP(150). Multinucleated cells were present adjacent to the newly-formed bone. These findings suggested that sufficient interconnection size, large enough for monocyte infiltration, may be an important factor for graft materials to express osteoinduction.
