2005 Volume 19 Pages 71-76
Adsorption properties of hydroxyapatite (HA) for acidic and basic proteins has been recognized to be improved by electrically polarization. In this study, adsorptions of bovine serum albumin and lysozyme to powders of the polarized HA using column chromatography were examined. Based on SEM observation, the morphologies of the polarized HA powders immersed in 0.01M phosphate buffer solution at pH 7.40 and 6.60 revealed that the dissolution of the powders was negligible for adsorption evaluation. The adsorption abilities of the polarized HA powder were significantly higher than those of non-polarized HA, in the pH ranging from 6.60 to 7.40, regardless of the adsorbate
