COMPLEX FORMATION OF BOVINE SERUM ALBUMIN WITH SODIUM DODECYL SULFATE ON THE SURFACE OF HYDROXYAPATITE

Abstract

Interaction among bovine serum albumin (BSA), sodium dodecyl sulfate (SDS), and hydroxyapatite (HAP) was studied in a phosphate buffer solution(pH 7.0) containing 0.02% sodium azide as an antiseptic at 25°C. Both BSA and SDS were simultaneously adsorbed on the surface of HAP. Adsorption amount of SDS in its low concentration range was higher in the presence than in the absence of BSA due to the complex formation between them on the surface of HAP. On the contrary, that of BSA decreased and levelled off after attaining a maximum with an increase in a concentration of SDS. The increase in the adsorption amount of BSA is by virtue of the formation of SDS-BSA complex on the surface. The decrease is owing mainly to the expansion/denaturation of BSA molecule through the complex formation with much amount of SDS on the surface of HAP.