PHOSPHORYLATION OF ADENOSINE 5'-MONOPHOSPHATE (5'-AMP) WITH CYCLO-TRIPHOSPHATE AND PURIFICATION OF THE PHOSPHORYLATED PRODUCTS
1995 Volume 5 Pages 137-142
Details
1995 Volume 5 Pages 137-142
Unprotected adenosine 5'-monophosphate (5'-AMP) was easily phosphorylated at the cis-2', 3'-diol by sodium cyclo-triphosphate (P3m) to form adenosine 2', 5'-bis (monophosphate) (2', 5'-ADP) and adenosine 3', 5'-bis (monophosphate) (3', 5'-ADP). The optimum condition for the phosphorylation of 5'-AMP with P3m is the mixing ratio of P3m to 5'-AMP (5:1), pH12, and room temperature. The yields of 2', 5'-ADP and 3', 5'-ADP were 47.2 and 49.3%, respectively, under the optimum condition. Purification of 2', 5'-ADP and 3', 5'-ADP was carried out with the fractional precipitation method.
