ADSORPTION OF BOVINE SERUM ALBUMIN ON AMORPHOUS CALCIUM PHOSPHATE IN RINGER SOLUTION

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The present study was made to investigate adsorption of bovine serum albumin (BSA) on hydroxyapatite (HAp) which was crystallized by dipping amorphous calcium phosphate (ACP) in Ringer solution having a composition similar to body fluid in vivo. Characterization of products was determined by means of X-ray diffraction, ion chromatography and ultraviolet spectrophotometry. When ACP was dipped for 48 hours in Ringer solution, maximum amount of BSA adsorbed on HAp prepared by crystallizing ACP was 190mg/g. Adsorption velocity till reaching to maximum amount of BSA adsorbed on HAp was speeded up by increasing concentration of BSA. The equilibrium amount of BSA adsorbed on HAp was 1.2mg/m². When ACP was dipped in Ringer solution, the largest quantity of BSA could be adsorbed to HAp crystallized in a short time.