消毒における水中RNAウイルスの損傷の検出

抄録

Disinfecting characteristics of viruses in water treatment with chlorine and TiO₂ photocatalyst were studied. RNA coliphage Qβ and poliovirus type 1 were used as model viruses. Viral inactivation was measured using plaque assay technique. Two kinds of RT-PCR methods combined with the MPN method were used to determine viral RNA damage. The SDS-PAGE was applied to determine the degradation of viral protein molecules. According to RT-PCR values, the viral RNA was damaged with 0.9 mg Cl/l free chlorine but not significantly damaged with 0.3 mg Cl/l combined chlorine concentration. Though RT-PCR results for both of Qβ and poliovirus showed no damage with a combined chlorine concentration of 0.3 mg Cl/l, according to plaque assay results, Qβ was inactivated llog in 20min. The results of SDSPAGE showed that Qβ protein molecules were degraded only with 400 mg Cl/l free chlorine concentration but not with 400mg Cl/l combined chlorine concentration. By TiO₂ photocatalyst combined with UV irradiation, the results of RT-PCR for Qβ showed Hog decrease in 3min, but showed no decrease even in 12min with UV irradiation alone.