Characteristics of organic wastes decomposition in hydrogen fermentation were investigated using batch reactor at35 °C Bean curd manufacturing waste, rice bran and wheat bran were used as the organic wastes. The organic wastes weresoaked in distilled water for dissolving carbohydrate and protein, and then the solution containing carbohydrate and protein wasused as the substrate for batch experiment. Although carbohydrate was rapidly consumed during hydrogen fermentation, nodecomposition of soluble protein was observed. It was suggested that the most of hydrogen was produced from carbohydratedecomposition. Hydrogen-producing microflora easily decomposed soluble carbohydrate of 46, 000 molecular weight. Hydrogenyields of bean curd manufacturing waste, rice bran and wheat bran were 2.54, 1.29 and 1.73 mol H2/mol hexose, respectively.Volatile fatty acids and alcohols were produced as the by-products from decomposition of substrate. Acetate, butyrate, ethanol, 2-propanol and 2-pentanol were the main substances.