脱嚢-フローサイトメトリー試験から観たCryptosporidium parvumオーシストの生存力に及ぼす水温の影響

抄録

The survival of the Cryptosporidium oocysts are affected in water temperature and storage time. We evaluated the survival of Cryptosporidium parvum oocyst under the conditions of controlled temperature from 4 to 60°C The survival ratio of oocysts was obtained using in vitro excystation-flow cytometry assay. As the result, the survival ratio of the oocysts rapidly decreased with the rise in water temperature. The required time for the decrease in the oocysts survival ratio of 90% at 60, 55, 50, 45, 40, 30 and 20°C was 3 min, 42 min, 3 hours, 31 hours, 7 days, 31 days and 150 days, respectively. The decrease of the survaival ratio of the oocysts at 10 and 4 °C was less than 10% after 150 days of storage.