Abstract
In order to detect enteric viruses from tap water and river water, a new virus concentration method wasdeveloped. Poliovirus type 1, LSc 2ab Sabin strain, was used as a model, and the concentration of poliovirus in waterwas determined by plaque assay using BGM cell line. We used a “cation-coated membrane, ” a negatively chargedmembrane coated with multivalent cations, for adsorption of viruses. For many enteric viruses have negative chargesin environmental samples, and they adsorb to a positively charged membrane. The procedure of rinsing this membraneby H2SO4 solution was applied for removing only multivalent cations, and poliovirus on the membrane was eluted by Na OH solution. By using a Millipore HA membrane and AlC13 solution, over 90% of poliovirus was recovered fromboth Milli Q water and tap water, while the recovery ratio from river water was low and unstable. This new methodenables us to know the behavior of enteric viruses in tap water.
