抄録
Outbreaks of waterborne infectious diseases caused by pathogenic viruses have been frequently documented over the world. Pathogenic viruses can survive for a long period in water environment, but there has been a difficulty in removing or inactivating viruses in conventional water and wastewater treatment systems. A new technology for the virus removal from water needs to be developed. In our previous study, virus-binding proteins (VBPs) demonstrating the ability to strongly bind Poliovirus type 1 (PV1) were recovered from a bacterial culture derived from activated sludge. It was expected that the isolated VBPs would be useful as viral adsorbents under the conditions of water and wastewater treatments. In order to develop new technology for virus removal, it is important to isolate not only VBPs for PV1 but also VBPs for other important pathogenic viruses in water. In this study, Adenovirus-Binding Proteins (ADVBPs) for Adenoviruses type 3 (AD3) and type 40/41 (AD40/41) were newly isolated from activated sludge culture with the affinity chromatography technique. Three distinct peaks were sequentially obtained in each affinity chromatographic profile of ADVBPs for AD3 and AD40/41, respectively. The isolated ADVBP in the first peak include a number of proteins, and their molecular weights were widely distributed. ELISA revealed that the ability of binding Adenoviral particle of ADVBP in the second and third peaks were stronger than those in the first peak in the affinit chromatographic profile. It is speculated that the isolated ADVBPs can be useful as specific adsorbents for Adenoviruses in several fields including virus removal, concentration and detection.
