Fabrication of Micro Fluidic Device for Blood Cell Count by Laser

Abstract

A number of particle detection devices have appeared in the micro total analysis system (μTAS) literature in recent years. Many of these devices mimic the operating principle of a bench-top flow-cytometer (FC) but on the micro-scale. The advantages of a μFC (micro flow-cytometer) are the requirement for extremely small sample volumes, high-speed testing, and economical. In this paper we present a novel approach to achieve rapid blood cell counting. The method uses a combination of 3-D laminar flow and embedding optical fibers in micro channels to achieve running cells in a row and detection. A laser fabricates a part of the channel on each film for every lamination, and then a 3-D micro-channel structure is fabricated.