Abstract
Application of micro patterning of phospholipids on an electrode and electroformation technique has an advantage of generation of size dispersed liposomes. In this paper, a new micro-fabrication method of micro spots on a Teflon film (Teflon AF , Dupont) was described to realize such phospholipids micro-patterning. This new method was based on the two techniques. The one was that the photoresist (OFPR-800, TOKYO OHKA KOGYO CO., LTD.) was baked before spin-coating. The other was that Teflon surface without photo resist film was fabricated by the cavitations effect using ultrasonic bath. In the traditional micro-fabrication methods of Teflon film, surface metallization or surface modification was needed; therefore, these processes led to the heavy environmental loads or the wettability change of Teflon film. In this new method, the wettability of the Teflon was kept during the process (The contact angles of distilled water and paraffin oil on Teflon was 114° and 70°, respectively.). Then the phospholipids were put on the spots, and a micro-patterned phospholipid on an ITO glass was realized. Giant liposome was generated using Electroformation method (10Hz, 5Vp-p)on it. This process will contribute to reduce the effects on the environment.
