Fabrication of Single Cell Sensor to Monitor Cell Metabolites

Abstract

Methods to rapidly analyze the single cell metabolic activity is a vital way to study in detail about the body dysfunction, cell proliferation, cell survival and carcinogenesis towards particular drug intake or any kind of diagnosis. As a main task of this project, we plan to fabricate three electrodes (working, reference and counter) in a single microneedle about the tip diameter of 1 μm - 3 μm and length about 3 mm for identification of several metabolites in single cell. A combination of electro polishing (to create needle) and sputtering (to deposit electrodes) will be employed to fabricate microneedle electrodes.