抄録
The mitogen (PHA and ConA)-induced lymphocyte blastogenesis was determined with 15 obese cynomolgus monkeys who were classified into spontaneously occurred obese with hyperlipamic and/or diabetes. There was no difference in the blastogenesis induced by PHA and ConA between obese and normal monkeys when their lymphocytes were cultured in medium supplemented with fetal calf serum. The blastogenesis of obese monkey lymphocytes, however, were significantly lower than that of normal monkey in the case of culturing them in the medium supplemented with autoserum. The wide variation was observed in blastogenesis of normal monkey lymphocytes when they were cultured with obese monkey serum, and some of obese monkey sera significantly inhibited blastogenic response of normal monkey lymphocytes. These results indicate that the responsiveness to mitogensin obese monkey lymphocytes might be normal but their serum might inhibit blastogenesis. Then, the relationship was determined between inhibitory effect of serum and contents of several biochemical markers associated with obese. The significant negative correlation was observed between inhibitory effect and total cholesterol as well as low-density cholesterol (VLDLC+LDLC). This result indicates that the level of serum LDLC+VLDLC possibly correlates with the inhibitory effect, and the increase of serum LDLC+VLDLC with obese might depress the lymphocyte function, resulting in the decrease of immune function in obese patients.
