Abstract
In order to examine the dynamic properties of osteoclast activity, we observed their motile events under the cultured condition by using video-enhanced DIC microscope system. Osteoclasts were isolated from the long bones of the rabbit, and placed on coverslips coated with calcium phosphate. The cells were incubated in the chamber on the DIC microscope at 35 degrees without CO2 supplement, and observed sequentially every 3 s for 60 min. Osteoclasts showed filopodial and lamellipodial movements on the calcium phosphate coated glass. Surprisingly, a part of filopodia and lamellipodia fragmented and transported the substrate of calcium phosphate. Occasionally, vacuoles were formed in the central area of the cell body. These results suggest that the osteoclast destructs the bone mechanically by filopodial and lamellipodial activity in the peripheral area. [Jpn J Physiol 54 Suppl:S84 (2004)]
