抄録
The present study was carried out to investigate whether angiotensin II (ANG II) peptide is induced in the rat's kidney under endotoxemic conditions. Immunohistochemical studies revealed that strong ANG II-like immunoreactivity was observed in the renal tubules of rats which received intraperitoneal (i.p.) injection of a high dose of LPS (1,000 μg/kg). Although ANG II-like immunoreactivity was slightly observed at 1 h after the injection of LPS, LPS-injected rats showed marked expression of ANG II-like immunoreactivity in the renal tubules at 3 h. There were few signals in the kidney of the saline-injected control rats. An i.p. injection of one of three doses of LPS (0.1, 10 or 1,000 μg/kg) produced dose-related increases in ANG II-like immunoreactivity in the renal tubules. Treatment with a prostaglandin-synthesis blocker, indomethacin, had no effect on the LPS-induced ANG II expression in the renal tubules. The ELISA study measuring the concentration of ANG II in the whole kidney supports the above mentioned findings. These results suggest that systemic administration of LPS results in the expression of ANG II peptide in the renal tubules, the mechanism being independent on the mediation by prostaglandins. [Jpn J Physiol 55 Suppl:S111 (2005)]
