抄録
Cold sensitive channel TRPA1 is activated by Noxious cold stimuli or pungent compounds, irritant drugs like isothiocyanate. But its active site is unkwon.Therefor, we measured the activity of variety of isothiocyanate to TRPA1 by using inner Ca2+ion concentration measurements,and study quantitative structure-activity relationship(QSAR) method for estimate activity site.Each compound induce [Ca2+] rise with dose dependent manner.Ec50 and Emax of those compound showed various value.We applicate QSAR method both EC50 and Emax values and search activity site and physical property. [Jpn J Physiol 55 Suppl:S139 (2005)]
