Spatio-temporal analysis of network activity in dentate gyrus by sparse loading with Ca²⁺ indicator

Abstract

Monitoring neuronal activities from multiple neurons is essential for understanding neuronal network activities. While calcium imaging from a population of cells is an effective method to study the network dynamics of a neural structure, it has been difficult to image from densely packed structures, such as the granule cell layer of the dentate gyrus, due to overlap of the cells. We have developed a novel method to label multiple granule cells retrogradely with a Ca²⁺ indicator in rat hippocampal slices using Oregon Green 488 BAPTA-1 AM. Synchronized burst activities (0.3–1.4 Hz), which were induced by applying 50 μM 4-aminopyridine, were monitored extracellularly with a glass electrode placed at the granule cell layer in the dentate gyrus. During the burst activities, action potential-induced Ca²⁺ transients from multiple (4–12) granule cells were monitored simultaneously with a cooled CCD camera with single-cell resolution. Temporal structures of firing patterns from the multiple neurons were determined from Ca²⁺ imaging data. In each single burst event recorded from the extracellular electrode, each neuron fired synchronously within a 200 ms time window. The latency and its variance from the onset time of the single burst events to one of the Ca²⁺ transients were decreased over time (< 7.5 min). These results indicate that the temporal synchrony of the action potentials within the single burst event was enhanced as the burst activities proceeded. The progressive synchronization may be a key feature to make self-organizing network activities. [Jpn J Physiol 55 Suppl:S57 (2005)]