抄録
Purpose: We attempt to classify the retinal proteins displayed on two-dimensional (2-D) gels based on their time-dependent expression patterns, which we designate "proteomic trajectory ", along the postnatal developmental axis. Methods: Retinas of C57/B6 mice were collected at postnatal (P) days 1, 3, 5, 7, 9, 14, 21 and at the adult stage. After separating the proteins by 2-D gel electrophoresis, the gel images were analyzed by Progenesis workstation. Protein spots were quantified and normalized, and the proteomic trajectory along the developmental axis was obtained. The results were averaged from four independent experiments. The proteomic trajectories were clustered by self-organizing mapping (SOM) using GeneCluster2. For protein identification each spot was excised and subjected to in-gel digestion by trypsin, followed by peptide mass fingerprinting (PMF). PMF search and confirmation were performed by MASCOT. Results and Conclusions: We identified ca 400 proteins. These proteins can be clustered by SOM into four major types, each exhibiting characteristic proteomic trajectory: Juvenile-type, showing abundant expression in the early postnatal stages and declining along the maturation process; Transient-type, showing transient expression during the development; Adult-type, showing increased expression in the later stages of development toward maturation; and Constitutive-Type, being expressed relatively constant during the entire developmental stages. [J Physiol Sci. 2006;56 Suppl:S101]
