抄録
Heat shock proteins (HSP) are synthesized by cells of all organism resistance to internal and external cellular stresses including physical stress, metabolic stress, and disease. Exercise is a sufficient stimulus to induce and enhance the synthesis of HSP70 and prolonged endurance exercise also causes an inflammatory reaction. Exercise induced a transient elevation in circulating leukocytes, driven largely by a granulocytosis but also influenced by an increase monocytes and lymphocytes. Present study was designed to investigate the expression of HSP70 in human peripheral blood leukocytes after acute moderate intensity exercise in trained runners and untrained subjects. Ten male long distance runners (TR, n=10, 21.3 yrs) and untrained control subjects (UT, n=10, 22.4 yrs) participated in this study. Subjects ran on a treadmill for 1 hr at 70% of heart rate reserve (HRR). Blood were taken immediately before and immediately after exercise, and at 30 minutes after exercise. HSP protein was evaluated by immunoblotting. Baseline HSP70 protein levels in TR was significantly lower than that in UT. Although expression rate of exercise induced HSP70 in both groups were similar, but UT showed significant higher HSP70 levels versus TR after exercise and at 30 minutes after exercise. We conclude that 1 hr treadmill running at 70% HRR intensity (moderate to heavy) is a sufficient stimulus of leukocytosis, neutrocytosis, lymphocytosis, and HSP70 expression in leukocytes. Adaptation to training are observed in TR subjects [J Physiol Sci. 2006;56 Suppl:S227]
