抄録
It has been reported that treatment of water by granular kaolinite ceramics modified the physical and biological properties of water. It was shown that the treated-water was positively charged without any absorption-deposition phenomena to substances contained in water. The positive charge of the treated-water was indirectly affirmed by thermally stimulated depolarized current measurements and Nuclear Magnetic Resonance spectrometry analysis. In the present study, we investigated an antioxidative effect of treated-water employing fibroblast cell culture system and Vitamin C preservation test. After cells were cultured in ordinary culture medium until sub-confluent state, then the medium was changed to media dissolved in Milli Q water (MEM) or Milli Q water treated by granular ceramics (tMEM), with or without various concentrations of hydrogen peroxide. Treatment of water was done by vigorous agitation of water with ceramic granules in a plastic bottle. After incubation for 24 hrs, the survival rate of the cells cultured in tMEM was significantly high compared to that of the cells cultured in MEM. Dissolved Vitamin C loses its reducing activity in time course. However, the reducing activity of Vitamin C in tMEM was significantly higher than that of Vitamin C in MEM, after prolonged incubation of the solution, suggesting the preventive effect of the treated-water against auto-oxidation of Vitamin C. Although the exact mechanism involved in the cell resistance against oxidative stress is not clear yet, it is strongly suggested that the treatment of water by granular ceramics gave antioxidative property to the water. [J Physiol Sci. 2006;56 Suppl:S238]
