抄録
We developed enhanced green fluorescent protein (eGFP) monitoring system which is able to measure eGFP that appeared to the living cell by the optical fiber, continuously. This system irradiates the excitation light to the neuron and the nerve ending where eGFP exists and the optical fluorescent signal is converted into the electric signal. In this study, using this system, we continuously measured change of fluorescence in arginine vasopressin (AVP)-eGFP transgenic rat posterior pituitary (PP) with several physiological stimulations. High potassium solution, salusinβ, and galanin-like peptide (GALP) showed a marked decrease of fluorescent signal that suggested AVP secretion from the nerve endings in the PP. These results suggest that this monitoring system has the possibility that AVP secretion can be indirectly monitored, as index the change of fluorescence. [J Physiol Sci. 2007;57 Suppl:S173]
