抄録
Albumin has a thiol group, called HMA (reduced form) when it is a free state. In contrast, albumin in which the thiol group is oxidized is called HNA (oxidized form). The major HNA is mixed disulfide with cystine (HNA-1), while the other is more oxidized state (HNA-2). We have studied the albumin thiol oxidation and the serum protein carbonyl formation in patients with chronic kidney disease (CKD). In CKD, the stage is classified by estimating the value for creatinine clearance using the Cockcroft-Gault formula (stage 1 to 5). In this study, 25 patients with CKD were divided into four groups (group A = stages 1 and 2; group B = stage 3a; group C = stage 3b; group D = stages 4 and 5). While the stage was advanced, mean value for HMA fraction was progressively decreased from 79.0 (group A) to 65.3% (group D) and that for HNA fraction was increased from 21.0 (group A) to 34.7% (group D). Serum protein carbonyl content was also progressively increased from 0.73 (group A) to 1.09 nM/mg (group D). In relationship between the thiol oxidation and the carbonyl formation, value for carbonyl content was not correlated with that for HNA-1 (R = 0.35, P = 0.09) but significantly with that for HNA-2 (R = 0.58, P <0.005). These results suggest that the measurements for albumin thiol oxidation and serum protein carbonylation in patients might be a useful biomarker in advancing stages of CKD, such as progressing renal dysfunction. [J Physiol Sci. 2007;57 Suppl:S217]
