抄録
We have identified gene expression changes in the paraventricular nucleus (PVN) and subfornical organ (SFO) of adult male Sprague Dawley rats following food or water deprivation. We interrogated Affymetrix microarrays with targets derived from the PVN or SFO of control, 2 day food deprived or 3 day dehydrated rats, and identified transcripts that are significantly up- or down-regulated a consequence of these stresses (Welsh t-test, p<0.05, n=5, with Benjamini-Hochberg multiple test correction and an arbitrary 2-fold change cut-off). Of the 127 transcripts that alter their expression in the PVN following dehydration, only 8 are also regulated in the SFO, out of a total of 259. In contrast, 900 genes are regulated in both the PVN and SFO by fasting, out of totals of 3515 and 1805 respectively. In the SFO, 234 transcripts alter in abundance with both dehydration and starvation, whilst 43 are similarly regulated in the PVN. Whilst most transcripts are similarly up- or down-regulated by both stimuli, some are regulated in the opposite direction–in the PVN, the gonadotrophin inducible transcription factor 1 (Giot1) mRNA is up-regulated by dehydration, but is apparently down-regulated by starvation. These data reveal that the physiological challenges of food and water deprivation result in clear and specific changes in gene expression in the PVN and SFO. We suggest that these changes may contribute to the integrated autonomic and neuroendocrine responses to these stressors. [J Physiol Sci. 2008;58 Suppl:S42]
