Cytogenetic Biodosimetry in Radiation Emergency Medicine: 6. Cytokinesis-block Micronucleus Assay and Its Role in Biodosimetry

抄録

An alternative method to dose estimation in cytogenetic biodosimetry is the cytokinesis-block micronucleus (CBMN) assay. Similar to the dicentric chromosome assay, this method correlates micronuclei formation in binucleated cells with absorbed dose. Instead of colcemid to arrest cells transiting from metaphase to anaphase, cytochalasin B is added to dividing human peripheral blood lymphocytes for cytokinesis inhibition. In this series, the history, applications, and recent developments of the CBMN assay in the context of cytogenetic biodosimetry are discussed. The topics covered include cell culture techniques, harvest and fixation processes, cell staining, imaging methodologies, micronucleus scoring criteria and procedures, and dosimetry applications. This review aims to provide insights into the various aspects of the CBMN assay, highlighting its significance and potential improvements for precise dose estimation in radiation exposure scenarios.