RADIOISOTOPES
Online ISSN : 1884-4111
Print ISSN : 0033-8303
ISSN-L : 0033-8303
Articles
Imaging Analysis of Direct Alanine Uptake by Rice Seedlings
Naoto NIHEISayaka MASUDAHiroki RAITomoko M. NAKANISHI
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2008 Volume 57 Issue 6 Pages 361-366

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Abstract
We presented alanine, a kind of amino acids, uptake by a rice seedling to study the basic mechanism of the organic fertilizer effectiveness in organic farming. The rice grown in the culture solution containing alanine as a nitrogen source absorbed alanine approximately two times faster than that grown with NH4+ from analysis of 14C-alanine images by Imaging Plate method. It was suggested that the active transport ability of the rice seedling was induced in roots by existence of alanine in the rhizosphere. The alanine uptake images of the rice roots were acquired every 5 minutes successively by the real-time autoradiography system we developed. The analysis of the successive images showed that alanine uptake was not uniform throughout the root but especially active at the root tip.
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© 2008 by Japan Radioisotope Association
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